Goal of the analysis

Analyzing qPCR data. Using Cq values from quantstudio application. Convert to absolute quantification using standard curves

## [1] "Using Standard curve : Std30"

Cq Plots

## Warning: Removed 9 rows containing missing values (geom_point).

## Warning: Removed 9 rows containing missing values (geom_point).

Copies plot

## Warning: Removed 9 rows containing missing values (geom_point).

## Warning: Removed 11 rows containing non-finite values (stat_boxplot).
## Removed 9 rows containing missing values (geom_point).

## Warning: Removed 11 rows containing non-finite values (stat_boxplot).
## Removed 9 rows containing missing values (geom_point).

## Warning: Removed 11 rows containing non-finite values (stat_boxplot).
## Removed 9 rows containing missing values (geom_point).

## Warning: Removed 11 rows containing non-finite values (stat_boxplot).
## Removed 9 rows containing missing values (geom_point).
## Warning: Removed 11 rows containing non-finite values (stat_boxplot).
## Warning in is.na(ticktext): is.na() applied to non-(list or vector) of type 'expression'

## Warning in is.na(ticktext): is.na() applied to non-(list or vector) of type 'expression'

## Warning in is.na(ticktext): is.na() applied to non-(list or vector) of type 'expression'
## Warning: Removed 11 rows containing non-finite values (stat_boxplot).
## Warning in is.na(ticktext): is.na() applied to non-(list or vector) of type 'expression'

## Warning in is.na(ticktext): is.na() applied to non-(list or vector) of type 'expression'

## Warning in is.na(ticktext): is.na() applied to non-(list or vector) of type 'expression'

Melting temp

Tm1

Largest melting temperature peak

All Tm peaks

All peaks of melting temperature

Data output

Mean data
Target_name assay_var.label mean_Copies.per.ul.template Sample_category
16s 328/0 4.21e+08 degradation
16s 328/10 4.46e+08 degradation
16s 328/30 4.22e+08 degradation
16s 328/60 4.28e+08 degradation
16s 328/180 3.74e+08 degradation
16s 315/0 3.21e+08 degradation
16s 315/10 4.91e+08 degradation
16s 315/30 4.13e+08 degradation
16s 315/60 3.24e+08 degradation
16s 315/180 2.42e+08 degradation
16s ntc 2.02e+02 Stability
gfpbarcode 328/0 1.74e+07 degradation
gfpbarcode 328/10 6.77e+05 degradation
gfpbarcode 328/30 2.28e+05 degradation
gfpbarcode 328/60 2.17e+05 degradation
gfpbarcode 328/180 3.45e+05 degradation
gfpbarcode 315/0 7.56e+07 degradation
gfpbarcode 315/10 1.13e+07 degradation
gfpbarcode 315/30 2.44e+06 degradation
gfpbarcode 315/60 1.18e+05 degradation
gfpbarcode 315/180 1.10e+04 degradation
gfpbarcode ntc NA Stability
U64 328/0 1.11e+03 degradation
U64 328/10 2.03e+02 degradation
U64 328/30 1.88e+01 degradation
U64 328/60 4.77e+00 degradation
U64 328/180 1.32e+00 degradation
U64 315/0 NA degradation
U64 315/10 NA degradation
U64 315/30 NA degradation
U64 315/60 NA degradation
U64 315/180 NA degradation
U64 ntc NA Stability